亚洲欧美国产vr在线观,暗交小拗女一区二区三区,国产熟女出轨做受的叫床声,国产999热这里只有精品

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CCL-171MRC-5 人胚肺細(xì)胞

MRC-5 人胚肺細(xì)胞

簡(jiǎn)要描述:CCL-171 MRC-5 人胚肺細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CCL-171
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-27
  • 訪  問  量:2211

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-171 MRC-5 人胚肺細(xì)胞

ATCC® Number:  CCL-171™

Designations:  MRC-5

Depositors:   JP Jacobs

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

CCL-171 MRC-5 人胚肺細(xì)胞

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: September, 1966

Applications: efficacy testing [92587]

testing [92346] [92389]

transfection host (Roche FuGENE® Transfection Reagents)

viruscide testing [92579] [92582]

Virus Susceptibility: poliovirus 1; herpes simplex; vesicular stomatitis (Indiana)

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,14

D16S539: 9,11

D5S818: 11,12

D7S820: 10,11

THO1: 8

TPOX: 8

vWA: 15

Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal.

Isoenzymes:  G6PD, B

Age:  14 weeks gestation

Gender:  male

Ethnicity:  Caucasian


Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

CCL-171



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
保德县| 凤山市| 通州市| 金阳县| 河北区| 津市市| 军事| 茌平县| 双峰县| 和平县| 陆河县| 武城县| 涞水县| 介休市| 故城县| 句容市| 余姚市| 遂川县| 武邑县| 万宁市| 东乌珠穆沁旗| 绍兴市| 贡山| 定安县| 土默特左旗| 花莲市| 双鸭山市| 东乌| 海兴县| 南阳市| 湖南省| 新余市| 获嘉县| 青州市| 弋阳县| 土默特左旗| 鸡西市| 怀宁县| 葵青区| 孟村| 新安县|